Fig. 12.

Induction of early and late apoptosis by Müllerian inhibiting substance (MIS) in endometrial stromal cells. Cells were treated with 71 nM MIS for 72 hours. For apoptosis, the externalization of phosphatidylserine was assessed by measuring annexin-V-FITC binding using propidium iodide as a counterstain. Quadrant rectangular dot grams from a representative of 3 independent experiments are shown (From Namkung J, et al. J Clin Endocrinol Metab 2012;97:3224-30, with permission from The Endocrine Society) [118].