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. 2014 Sep 1;13:204. doi: 10.1186/1476-4598-13-204

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© Mohammad et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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MCD augments antiproliferative activity of tamoxifen on tumor cells in vitro . Combination of tamoxifen and MCD exerts synergistic effect on different cancer cell lines. (A-B) A375 and (D-E) B16F10 cells were treated with indicated concentrations of tamoxifen together with MCD for 24 h. Data were presented as percentage of cell proliferation measured by MTT assay. (C and F) A375 and B16F10 cells respectively were treated with indicated concentration of tamoxifen along with MCD. Long term clonogenic survival assay was performed and colonies were assessed by Image J software. (G and H) MCF-7 and HeLa cells respectively were treated with indicated concentrations of tamoxifen together with MCD for 24 h. Data were presented as percentage of cell proliferation measured by MTT assay. Bar graph represents the Mean ± SD of an experiment done in triplicate (*P ≤ 0.05, **P ≤ 0.001, ***P ≤ 0.0001).