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. 2014 Sep 1;13:204. doi: 10.1186/1476-4598-13-204

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© Mohammad et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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MCD enhances the susceptibility of A375 and B16F10 cells to tamoxifen by alteration in cell cycle and DNA fragmentation. A375 and B16F10 cells were treated with indicated concentration of tamoxifen along with MCD. (A and D) Display of the percentage cells in different phases of cell cycle by flow cytometry. (B and E) The treated cells were assayed by DNA fragmentation. (C and F) Whole cell lysate were subjected to Western blotting and expression level of PARP, Bcl-2 and Bax was detected. GAPDH served as a loading control. Relative folds (RF) values are expressed in arbitrary unit. Data are representative of experiments performed in triplicate.