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. 2014 Sep 1;13:204. doi: 10.1186/1476-4598-13-204

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© Mohammad et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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MCD treatment enhances the susceptibility of cells to tamoxifen induced cell death and cholesterol treatment reverses the effect. (A and D) A375 and B16F10 cells were treated with indicated concentration of tamoxifen in- combination with MCD and cholesterol. Data were presented as percentage of cell proliferation measured by MTT assay. (B and E) Long term clonogenic survival assay. (C and F) Relative quantitation of colonies by Image J software in different fields. Bar graph in MTT assay represents the Mean ± SD of experiments done multiple times in triplicate (*P ≤ 0.05, **P ≤ 0.001, ***P ≤ 0.0001).