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. 2014 Sep 12;15(1):788. doi: 10.1186/1471-2164-15-788

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© Tallon et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Assembly differences. The differences between the HGAP2 assembly presented here and the prior 454-based assembly are examined using NUCMER and BLASTN. The larger number of sequences uniquely in the HGAP2 assembly and the larger number of low complexity sequences suggests that the PacBio sequence data resolves more of the genome by spanning low complexity repeats. Some regions were identified in the 454-based assembly that were in the Illumina MiSeq assembly, suggesting that the sequences are missing from the HGAP2 assembly.