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. 2014 Sep 12;15(1):788. doi: 10.1186/1471-2164-15-788

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© Tallon et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Sequencing coverage distribution. A histogram of the coverage at each position following mapping of the Illumina MiSeq reads to all assemblies are plotted with log-transformed axes. Regions with abnormally little coverage exist, as well as those with excess coverage, for all assemblies. The peak in coverage for the Illumina data was at 51× coverage. In addition to this major peak, two additional peaks were identified at 200× and 450× in the HGAP2 assembly, reflecting collapsed repeats in the assembly including the mitochondrial genome, a L. loa interspersed repeat, and the rRNA.