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. 2014 Sep 12;15(1):788. doi: 10.1186/1471-2164-15-788

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© Tallon et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Relationship between coverage and percent heterogeneity. A SNP was defined as a position with >20× coverage that had polymorphisms supported by >3 reads. For each position with a SNP, the percent heterogeneity and coverage are plotted. The red line shows the minimum bounds of the data that is defined by requiring >20× coverage and polymorphisms in three reads. As expected, some of the 100% heterogenous positions have higher coverage than average suggesting that they may be collapsed repeats. This plot does not enable any conclusions about the frequency at which a value occurs since data points with the same values are only plotted once.