Table 1.
Effects of single and combined metal ions and H2O2 on U937 cell number and viability.
| Reagent | Viability (%)a | Cell no. (107well)b |
|---|---|---|
| Ni2+ at (20µM) | 96.4 ± 0.3 | 5.4 ± 0.24 |
| Cu2+ at (20µM) | 97.2 ± 0.4 | 5.7 ± 0.26 |
| Ni2+ + Cu2+ at (5 µM) | 95.4 ± 0.2 | 5.5 ± 0.15 |
| Ni2+ + Cu2+ at (10 µM) | 98.2 ± 0.2 | 5.3 ± 0.15 |
| Ni2+ + Cu2+ at (20 µM) | 97.1 ± 0.5 | 5.7 ± 0.56 |
| H2O2(0.01mM) | 95.6 ± 0.8 | 5.2 ± 0.25 |
U937 cells were incubated at a density of 1.8 × 106 cells/well. Each table in this and other tables in this article represent means for four different experiments for each dose level of metal tested, which was assayed in triplicates.
a
Viability (measured by trypan blue exclusion) was determined before (96.8 ± 0.25%)
b
cell number (i.e., growth) after incubation with the above reagents at 37°C for 24 hours.