Table 2.
Levels of GSH in untreated and treated U937 cells with the individual reagents.
| Reagent | GSH level (nmoles/106 cells)a |
|---|---|
| Untreated cells | 10.38 ± 0.42 |
| H2O2(0.01mM) | 9.76 ± 0.21 |
| Ni2+ at (20µM)b | 10.15 ± 0.52 |
| Cu2+ at (20µM)b | 8.30 ± 0.71 |
| Ni2+ + Cu2+ at (5 µM) | 7.99 ± 0.23 |
| Ni2+ + Cu2+ at (10 µM) | 9.21 ± 0.15 |
| Ni2+ + Cu2+ at (20 µM) | 10.20 ± 0.44 |
U937 cells were incubated at a density of 2.1 × 106 cells/well. GSH levels were analyzed after incubation without and with the above individual reagents at 37°C for 24 hours.
a
GSH levels for the different doses of reagents employed were not different from each other; hence, the values for the high doses were reported for the single metal ions.