. Author manuscript; available in PMC: 2014 Sep 26.

Published in final edited form as: Drug Chem Toxicol. 2012 May 27;36(2):155–162. doi: 10.3109/01480545.2012.660947

Table 3.

Effect of EDTA on GSH in U937 cells.

Ni²⁺ (µM)	EDTA (50 µM)	EDTA (100 µM)	Cu²⁺ (µM)	EDTA (50 µM)	EDTA (100 µM)	Ni²⁺ + Cu²⁺(µM)	EDTA (50 µM)	EDTA (100 µM)
0	11.4 ± 0.3	10.4 ± 0.3	0	10.7 ± 0.3	11.8 ± 0.3	0	12.6 ± 0.3	11.4 ± 0.3
5	8.2 ± 0.4^*	10.2 ± 0.4	5	9.2 ± 0.4	8.2 ± 0.4	5	8.2 ± 0.4^*	9.2 ± 0.4
10	6.8 ± 0.2^**	8.4 ± 0.2^*	10	7.7 ± 0.2^*	6.4 ± 0.2^**	10	6.4 ± 0.2^**	7.4 ± 0.2^**
20	5.2 ± 0.2^**	6.7 ± 0.2^**	20	5.8 ± 0.2^**	5.0 ± 0.2^**	20	5.2 ± 0.2^**	3.9 ± 0.2^**

U937 cells were incubated at a density of 1.8 × 10⁶ cells/well. GSH (nmole/10⁶ cells) levels were measured after incubation with the above reagents in the presence of 0.01 mM of H₂O₂ at 37°C for 24 hours. Statistical significances denoted by asterisk symbols are shown as a comparison between each control subgroup without metal ions (i.e., Ni or Cu) with treatment with EDTA at 50 or 100 µM and its treated subgroup with EDTA for the respective metal ions (i.e., Ni²⁺ or Cu²⁺ + 5, Ni²⁺ or Cu²⁺ + 10, and Ni²⁺ or Cu²⁺ + 20).

P < 0.05;

^**

P < 0.01;

^***

P < 0.001.