Figure 5. Inhibiting Asl function strongly perturbs DSpd-2 incorporation into the PCM.
Images (A–D) show results from FRAP experiments monitoring how DSpd-2-GFP (green) incorporation into the PCM is affected by inhibiting the function of various centriole-associated components with injected Texas-red-labelled antibodies (red—as indicated). The antibodies bind to their cognate protein at centrosomes close to the injection site (B–D), but not to those far from the injection site (A), which therefore act as internal controls. (E) Graph displaying the initial recovery rate of DSpd-2-GFP fluorescence at centrosomes bound with antibodies (as indicated below the graph) relative to the centrosomes not bound by antibodies (control). The rate of recovery was calculated by measuring the gradient of the initial linear phase of recovery that occurred over the first 60 s after photobleaching. Note how anti-Asl antibodies reduce the rate of DSpd-2-GFP incorporation into the PCM by ∼75%, but that anti-DSas-4 or anti-D-PLP antibodies have little or no effect. Error bars = standard error. See also Video 3.