Figure 6. A yeast-two-hybrid analysis examining the interactions between Asl, DSpd-2, and Cnn.
A schematic summary of the yeast two-hybrid interactions observed between Asl, DSpd-2, and Cnn. The shades of the lines indicate the strength of the interactions observed: strong (dark blue), medium (blue), and weak (light blue). The characteristics of the lines indicate the number of different positive reporter assays for each interaction: solid line (3/3 assays), large dashed line (2/3 assays), and small dashed line (1/3 assays). Arrows point from bait to prey fragments—double-headed arrows indicate that the interaction scored positive with either fragment as bait or prey. See Figure 6—source data 1.
DOI: http://dx.doi.org/10.7554/eLife.03399.014
Figure 6—source data 1. A yeast-2-hybrid analysis testing the interactions between various Asl, DSpd-2, and Cnn fragments.
(Related to Figure 6) A yeast-2-hybrid analysis was carried out using various bait and prey fragments of each protein (as indicated in columns A and B). Three different reporters were tested—His (columns C–F), Ade (columns G and H), and LacZ (column I). Interaction levels are indicated as strong, medium, weak, or none. All combinations of baits and preys were tested, but only those that scored positive in at least one assay are shown.
elife03399s001.xlsx (20.5KB, xlsx)
DOI: 10.7554/eLife.03399.015