Figure 7. The centrosomal levels of Cnn are strongly reduced in eggs lacking DSpd-2.
(A and B) Images show centrosomes co-stained with DSas-4 antibodies (red) and Cnn antibodies (green) in fixed eggs that either contained (A) or lacked (B) endogenous DSpd-2. As embryos lacking DSpd-2 fail in pronuclear fusion (Dix and Raff, 2007) (and so fail to develop), we induced the de novo formation of centrosomes in WT and DSpd-2 mutant eggs by over-expressing Sak kinase (Peel et al., 2007; Rodrigues-Martins et al., 2007). The centrosomal levels of Cnn are dramatically reduced in the absence of DSpd-2. (C) The graph quantifying the centrosomal levels of Cnn, DSpd-2, γ-tubulin, and α-tubulin in Sak over-expressing eggs that either contained (black bars) or lacked (white bars) endogenous DSpd-2. The centrosomal levels of Cnn, γ-tubulin, and α-tubulin are dramatically reduced in the absence of DSpd-2.