(A–D) Graphs show the average fluorescence intensities of interphase (blue dots) and mitotic (black dots) centrosomes from either WT (A), cnn mutant (B), dspd-2 mutant (C), or cnn;dspd-2 double mutant (D) larval brain cells stained for various centrosomal proteins (as indicated below graphs). Each data-point represents the average centrosome value from one brain. The horizontal red bars indicate the average value of all the brains. All the PCM proteins are still partially recruited to centrosomes in the absence of Cnn or DSpd-2 (with the possible exception of Aurora A, which does not appear to be recruited in the absence of DSpd-2). The mitotic PCM levels do not rise above interphase levels in the absence of both Cnn and DSpd-2, indicating that centrosome maturation has been abolished. (E–L) Images show typical mitotic cells from either WT (E and I), cnn (F and J), dspd-2 (G and K), or cnn;dspd-2 double mutant (H and L) larval brain cells stained for the centriole marker Asl (red), mitotic DNA (phospho-histone H3, blue), and either the PCM marker γ-tubulin (green, E–H) or MTs (I–L, green). Error bars = SEM. See also Figure 9—figure supplements 1 and 2.
DOI:
http://dx.doi.org/10.7554/eLife.03399.019