Video 3. The rate of DSpd-2-GFP incorporation into the PCM is reduced in embryos injected with anti-Asl antibodies.
(related to Figure 5). All Videos shown here are maximum intensity projections of image stacks. These videos show the fluorescence recovery of DSpd-2-GFP (green) at centrosomes in embryos that were injected with fluorescently labelled antibodies (red) against Asl (A, B), DSas-4 (C), or D-PLP (D). Time before and after photobleaching (t = 0 s) is shown at the top right of (D). An example of a centrosome in an embryo injected with anti-Asl antibodies that was located far from the injection site and so received a low concentration of the antibody is shown in (A); these centrosomes acted as internal controls. Panels (B–D) show examples of centrosomes located close to the injection sites of antibodies against Asl (B), DSas-4 (C), and D-PLP (D); these centrosomes received a high concentration of each antibody. Note that DSpd-2GFP fluorescence recovered at a much slower rate at centrosomes that bound anti-Asl, whereas DSpd-2GFP fluorescence recovered at near normal rates at centrosomes that bound anti-DSas-4 or anti-D-PLP antibodies.