Video 5. Centrosomal MTOC activity is only abolished in cells lacking both Cnn and DSpd-2.
(Related to Figure 9). All Videos shown here are maximum intensity projections of image stacks. These videos show the distribution of the centriole marker GFP-PACT (pseudo-coloured red) and the MT marker Jupiter-mCherry (pseudo-coloured green) in either WT (A), cnn (B), dspd-2 (C), or cnn;dspd-2 mutant neuroblasts as the cells progress through mitosis. Time before and after anaphase onset (t = 0 s) is shown at the top right of each panel. Note how the centrosomal MT asters can be observed in WT (A), cnn (B), and dspd-2 (C) mutant neuroblasts, and these MT asters appear to contribute to spindle assembly. No centrosomal MT asters, however, can be observed in cnn;dspd-2 double mutant neuroblasts and the spindle appears to form independently of centrosomes (D).