The top 5 WT Treg cell TCRs, of which the top 4 are BM APC-dependent, were retrovirally transduced into Rag1–/– thymocytes, which were intrathymically injected into WT, CD11c-Cre ROSA-DTA, or C2TAkd hosts. Data shown are representative flow cytometry plots (left) and summary graphs (right) with each dot representing the data from one host. Each TCR was analyzed in at least 2 independent experiments with 2-3 replicates. **p < .01, ***p < .001, ns = not significant (Mann-Whitney U). See also Figure S4.