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. Author manuscript; available in PMC: 2015 Mar 1.
Published in final edited form as: Nat Biotechnol. 2014 Jul 6;32(9):933–940. doi: 10.1038/nbt.2943

Figure 6.

Figure 6

roX1’s D domains are independent, functional RNA subunits. (a) Transgene designs. Transgenic constructs of full-length roX1 and the six individual domains were cloned, chromosomally integrated, and expressed under the tubulin-GAL4 promoter in roX-null flies. (b) Rescue of male lethality by roX1 transgenes. Transgenic males surviving to adulthood were counted and normalized to females. Only the D domains rescued males appreciably. Rescue by D3 is not significantly different from that of full-length roX1 (t-test, P-value=0.20). Average of three separate crosses +s.d. shown (on average, n=800). roX transgene expression was quantified and normalized to endogenous roX1 expression in wild-type males, represented as relative fold (transgene/endogenous) ±s.d. (c, d) Integrated interaction map of the dosage compensation complex with chromatin. (c) roX1 RNA is topologically organized such that the three U domains form a core palm and each of the D domains extends independently as a finger. Each D domain finger directly binds to proteins of the MSL complex, with domain D3 having the highest affinity and D1 the weakest. (d) CLAMP binds the GAGA motif at X-linked CES, and associates with MLE. MLE binds to stem-loops on roX1, which tethers MLE to the core MSL complex. MOF of the MSL complex recognizes and acetylates H4K16 of adjacent nucleosomes.