Figure 2. In vivo laser axotomy induces a dramatic rise in axonal Ca²⁺ that is suppressed by Wld^S.

(A) tdc2-Gal4 labels 3 axons in each peripheral nerve, only one segment is illustrated.

(B) Axons were labeled with mCD8::mCherry, axonal Ca²⁺ was monitored by co-expressing GCaMP3 in the tdc2-Gal4⁺ subset of neurons. Note the breakage of the axon after laser axotomy (red, mCherry). Axonal Ca²⁺ levels one minute after axotomy (green, GCaMP3).

(C,D) Representative traces showing Ca²⁺ responses in axon fragments distal to the injury site over time. Genotypes as indicated.

(E) Quantification of peak Ca²⁺ intensities and time to ½ recovery from average peak intensity for each genotype listed. n≥5 live samples for each genotype and time point versus control. *, p<0.05; **, p<0.01; ***, p<0.001.