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Location of the RL2 (ICP0) exon 2/3 splice junction. Predicted AA sequences are shown in the top 5 lines, with the shaded BVrh E2490 AA sequence being that originally reported [14]. DNA sequences for BVrh E2490 and BVcy E90-136 are shown in the two lower lines. PCR/sequencing of RL2 mRNA from the three BVrh strains and BVcy resulted in predicted splice junctions producing ICP0 AA sequences that are identical in all four BV strains.
