TABLE 3. Effects of oxidants and inflammatory stimuli on let-7a levels in HUVECs and mouse aorta.
| Treatment | Fold change ± SE | n | p |
|---|---|---|---|
| 10-μM acrolein | 1.42 ± 0.18 | 13 | 0.02 |
| 10-μM HNE | 0.97 ± 0.08 | 10 | >0.05 |
| 25-μM HNE | 0.66 ± 0.09 | 3 | >0.05 |
| 50-μM HNE | 1.01 ± 0.04 | 3 | >0.05 |
| 10-μM POVPC | 0.82 ± 0.08 | 3 | >0.05 |
| 10-μM acrylic acid | 0.51 ± 0.14 | 4 | 0.05 |
| 10-μM allyl alcohol | 0.80 ± 0.27 | 5 | >0.05 |
| 10-μg/ml ox PAPC | 0.80 ± 0.001 | 3 | 0.001 |
| 25-μg/ml ox PAPC | 0.84 ± 0.11 | 3 | >0.05 |
| 50-μg/ml ox PAPC | 0.59 ± 0.36 | 3 | >0.05 |
| 200-μM H2O2 | 0.92 ± 0.12 | 9 | >0.05 |
| 10-ng/ml TNF-α | 0.97 ± 0.14 | 5 | >0.05 |
| 1-μg/ml LPS | 1.08 ± 0.14 | 5 | >0.05 |
| Inhaled acrolein | |||
| (a) Intact aorta | 1.87 ± 0.25 | 25 | 0.04 |
| (b) denuded aorta | 1.26 ± 0.03 | 5 | >0.05 |
Levels of let-7a were measured in HUVECs after a 2-h treatment with the listed stimuli and a 4-h recovery period. For measurements in the aorta, the mice were exposed to 1-ppm acrolein or filtered air for 4 days and changes in let-7a were measured immediately after the end of the last exposure. The miRNA levels were measured by rtPCR. Values are fold change ± SE versus control.