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. 2014 Sep 26;8:104. doi: 10.3389/fnana.2014.00104

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Copyright © 2014 Botcher, Falck, Thomson and Mercer.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Definition of the borders of the CA2 region. (A) GAD67 fluorescent staining (scale bar 1 mm). GAD-positive neurons in the CA1, CA2, and CA3 regions are shown at higher magnification in the bottom panels (scale bars 200 μm). SO, Stratum Oriens; SP, stratum pyramidale; SR, stratum Radiatum; SLM, Stratum Lacunosum Moleculare; SL, Stratum Lucidum. (B) Immunoperoxidase staining of parvalbumin (PV)-positive interneurons (scale bar 1 mm). (C) Immunofluorescence staining for α actinin 2 protein and purkinje cell protein 4 (PCP4) determined the borders of the CA2 region. Scale bars, 1 mm. (D) The borders of CA2 (white dotted line) were defined by superimposition of the PCP4 labeling and GAD67 and immunoperoxidase stainings.