Skip to main content
. 2013 Oct 31;44(1):104. doi: 10.1186/1297-9716-44-104

Figure 3.

Figure 3

Growth and stability of rHP-PRRSV/SD16/TRS6-EGFP in Marc-145 cells. A. Marc-145 cells were infected with HP-PRRSV/SD16 or rHP-PRRSV/SD16/TRS6-EGFP and the amount of virus in the cultures was measured at various times from 12 to 96 h pi. B. rHP-PRRSV/SD16/TRS6-EGFP was passaged in Marc-145 cells every 3–4 days. The cells of each passage were freeze-thawed three times and used for virus titration. C. EGFP expression throughout virus passages in Marc-145 cells was analysed by quantitating relative fluorescence using a GFP Quantification Kit. Data were expressed as the mean ± standard deviation (SD).