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Genetic stabilization in Rev 2 does not alter the in vitro growth and vaccine properties of Rev 1 reference vaccine. (A) In vitro growth kinetics; (B) Residual virulence (log CFU/spleen); (C) Spleen weights; and (D) Efficacy against a virulent B. melitensis virulent challenge, in BALB/c mice. Statistical comparison of means was performed by one-way ANOVA and Fisher’s PLSD test: aP < 0.005 vs. unvaccinated control (PBS group).
