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Expression and purification of recombinant H11-4, H11-1 and H11-5 expressed in C. elegans. rH11 proteins purified by cobalt chelation chromatography were separated by 4-15% SDS-PAGE and stained with Coomassie blue or transferred to PVDF membrane and probed with anti-His (C-terminal) antibody or antiserum to native H11-enriched extract (Anti-nH11). Antiserum from animals prior to H11-extract immunisation showed no reactivity to rH11 isoforms.
