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Antibody reactivity to glycan epitopes on C. elegans recombinant or native H11 proteins. Western blots showing recognition of rH11-4, rH11-4ΔN-Gly, native H11-enriched extract and native H11-enriched extract treated with mild periodate, by antibody to native H11 extract (anti-nH11; panel A) or to rH11-4/5 proteins (anti-rH11; panel B). Reactivity of anti-His antibody confirmed equal loading of rH11-4 protein (panel C). Proteins in addition to H11 are observed in the nH11 extract.
