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. 2014 Jul 17;42(17):e130. doi: 10.1093/nar/gku616

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© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Time course of the titration of LexA-ER-AD activity. Strains containing LexA-ER-B42, LexA-ER-B112, LexA-ER-Gal4AD or LexA-ER-VP16 and a target promoter with four lexA boxes driving Citrine expression (FRY418, FRY666, FRY667, FRY743) were incubated in a concentration series of β-estradiol in SDC. At each time point, the induction levels were measured by flow cytometry. Experimental (symbols, mean ± standard deviation) and simulated (gray surfaces and contour lines) time-dependent dose-response are plotted against the logarithmic concentration of inducer.