Figure 5.

Induction of LexA-ER-B42 in different growth conditions. The induction levels of a prototroph strain containing LexA-ER-B42 and target gene with four lexA boxes in its promoter (FRY865) grown in SDC, SGlyC and SDP were measured by flow cytometry. Symbols represent the median and error bars the 25th and the 75th percentiles of the fluorescence signal (area) distribution measured. (A) Timing of the induction with 2000 nM β-estradiol. (B) Titration using a concentration series of β-estradiol. Cells were induced for 24 h. The x-axis is logarithmic.