Figure 4.

uORF and 5′ UTR coverage in ribosome profiling experiments. (A) There is a dramatic difference in cumulative 5′ UTR occupancy if cycloheximide treatment is omitted. (B) None of the three examined stresses significantly increase 5′ UTR ribosomal occupancy. Although oxidative stress and amino acid starvation do slightly increase 5′ UTR occupancy, the effect is minimal compared to what was previously found (1,2). (C) Addition of translation initiation inhibitors does not affect cumulative ribosomal occupancy at the 5′ UTR.