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. 2014 Aug 28;42(17):11071–11082. doi: 10.1093/nar/gku779

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© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 6. — Pols ι and κ are required for efficient bypass of photoproducts in MEFs deficient in Polη. (A) Wild-type MEFs (WT) or MEFs with single, double or triple deficiencies in Polη (η), Polι (ι) and Polκ (κ) were pulse labeled with EdU for 30 min, prior to exposure to 5 J/m² UVC, and 2 and 8 h later, MEFs were fixed and immunostained for CPD (online in green; upper panels) in ssDNA of nuclei (online in blue; lower panel) in replicating, EdU-incorporating (online in red, merged with CPD staining; middle panels) and non-replicating MEFs at the time of UVC exposure. (B) Similar as in (A) except that MEFs were immunostained for (6–4)PP (online in green; upper panel). Rev1-deficient MEFs (Rev1) were included as a positive control (15,16).