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. 2014 Sep 2;42(17):11218–11232. doi: 10.1093/nar/gku782

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© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — cMBD2_MBD does not exchange between binding sites as efficiently as MBD4_MBD. (a) The intensities for auto (AA and BB) and exchange (AB and BA) crosspeaks for Glu²¹ from N_z-exchange spectra were fit to four coupled equations to determine the rate of intermolecular exchange at 185 μM (upper panel) and 370 μM (lower panel) cMBD2_MBD concentration. (b) 2D ¹H-¹⁵N TROSY HSQC spectra of cMBD2_MBD bound to methylated wild-type and inverted (10 bp) DNA (red, upper panel), tandem (30 bp) DNA (blue, middle panel) and tandem (20 bp) DNA (orange, lower panel) show that intramolecular exchange for cMBD2_MBD remains in the slow to intermediate NMR timescale when bound to the tandem (30 bp) DNA. If the sites are 4 bp closer together as in the tandem (20 bp), then cMBD2_MBD exchanges between sites on the fast NMR timescale.