Table 3. DNA binding affinity.
KD (μM) | Rmax | X2 (10−4) | |
---|---|---|---|
MBD4MBD-mCpG | 6.4 ± 1.5 | 0.74 | 34 |
MBD4MBD-TpG | 11.5 ± 1.2 | 0.59 | 2.3 |
MBD4MBD-CpG | 17.2 ± 2.0 | 0.91 | 3.5 |
MBD4MBD-hmCpG | 14.2 ± 1.9 | 0.72 | 3.9 |
MBD4MBD(Y109F)-mCpG | 7.9 ± 1.6 | 0.63 | 14 |
MBD4MBD(Y109F)-TpG | 14.0 ± 1.4 | 0.51 | 1.0 |
MBD4MBD(Y109F)-CpG | 19.6 ± 3.5 | 0.76 | 3.5 |
MBD4MBD(Y109F)-hmCpG | 16.0 ± 3.0 | 0.59 | 3.4 |