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. 2014 Aug 28;42(17):11119–11135. doi: 10.1093/nar/gku783

Figure 2.

Figure 2.

WRN is modified by HNE in vivo. Human normal (WT) or WS fibroblasts were pre-treated with 100 μM HNE for 2 h. Cell extracts were prepared and immunoprecipitated with rabbit anti-WRN or mouse anti-HNE antibodies (lanes 6–8, A and B, respectively) or control IgG (lanes 4 and 5, A and B). The immunoprecipitates were analyzed by SDS-PAGE and western blot with mouse anti-HNE or mouse anti-WRN (A), or rabbit anti-WRN antibodies (B). Input, 2.5% loaded. Lanes 1, input from WT cell extract. Lanes 2, the same as lanes 1, but cells were pre-treated with HNE. Lanes 3, Input from WS cell extract pre-treated with HNE.