Figure 3.

Unwinding and cleavage of splayed arm substrates by Pcal_0546 and SSO1391. (A, B) Cleavage of splayed arm substrates by the indicated amounts of Pcal_0546 (A) or SSO1391 (B) incubated for 45 min (A) or 15 min (B) and analyzed by denaturing gel electrophoresis. In all substrates, one strand is [³²P]-labeled at the 3′-end (A) or 5′-end (B) (indicated by asterisks), and the length of ssDNA overhangs is indicated on the substrate models (nt). Lane C shows incubation without protein.