Table 2. F tests comparing the variance in mutational impact on intrinsic DNA flexibility (dTRX) for functionally conserved versus adaptively evolving genes (observed in Figure 7).

Species/group	F value syn	P value syn	F value non-syn	P value non-syn	n conserved	n adaptively evolving
Methanococcus maripaludis	5.13585	4.44 × 10−15	0.3902547	5.63 × 10−11	1169	77
Bacillus sp	6.863267	0	0.2741067	5.81 × 10−26	1565	94
Brucella-Ochrobactrum	8.410006	0	0.4192114	1.25 × 10−09	1534	77
Campylobacter sp	6.3016	5.30 × 10−08	0.3611305	2.31 × 10−06	869	31
Listeria sp	13.05	1.62 × 10−14	0.1765246	1.80 × 10−22	884	37
Mycobacterium sp	13.05	1.62 × 10−14	0.1765246	1.80 × 10−22	884	37
Nitrobacter sp	6.813388	1.38 × 10−09	0.5375341	0.003787364	709	36
Prochlorococcus marinus	3.568853	0	0.5972523	9.04 × 10−06	1045	147
Pseudomonas aeruginosa	3.263118	1.27 × 10−07	0.3782765	4.61 × 10−10	3142	59
Pseudomonas sp	8.341558	0	0.2492518	9.13 × 10−23	2495	63
Pseudomonas syringae	6.507828	0.000163719	0.3905284	0.002296555	409	15
Rhodobacter sphaeroides	6.981686	5.92 × 10−05	0.3803578	0.001223606	408	16
Rickettsia sp	14.15104	1.98 × 10−06	2.42576	0.05746677	607	14
Shewanella sp	4.076466	1.45 × 10−13	0.2152939	1.09 × 10−36	1770	87
Vibrio sp	5.911175	2.21 × 10−06	0.3008131	2.23 × 10−07	764	25
Yersinia sp	22.60118	0	0.1968196	7.17 × 10−23	1426	43

Tests were conducted independently for effect of synonymous and non-synonymous sites on dTRX across all aligned gene sets.