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. 2014 Sep 8;42(17):11011–11024. doi: 10.1093/nar/gku814

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© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Distribution of histones, Pol II, cdk9, Trap220 and p300 on the Plau locus. ChIPs were conducted in MDA-MB231 (MDA) and MCF7 (A–D and J) cells using antibodies specific for histone H3 (A), H3K4me3 (B), H3K9ac (C), RBP1- (Pol II) (D and H), phospho-Ser5 (E), phospho-Ser2 RBP1 CTD (F), cdk9 (G), Trapp220/Med1 (I) and p300 (J). (H) ChIP conducted using an antibody against RNA Pol II in MDA-MB231 cells treated, or not, with DRB (65 μM) for 4 h. Values are the mean of at least three experiments. For calculation of means, all values were normalized to that of amplicon −1.9 in MDA-MB231 cells, which was arbitrarily set to 1, except for (i) H3K4me₃ and H3K9ac where values were normalized to that of amplicon −1.3 set to 1 and (ii) P-S2 where values were normalized to that of amplicon 3.5 set to 1. In the case of (H), normalization was achieved with respect to control conditions. Bars indicate standard deviation. Results of the Student's paired t-test are indicated on the graphs.