FIGURE 4.

AT2R knockdown inhibits skeletal muscle regeneration in vivo. A, experimental model. siRNA electroporation was performed on day 0. Under this experimental condition, the electric pulse damages muscles and induces regeneration. Gastrocnemius muscles were collected on days 3, 5, and 7, and RNA was extracted. B–D, qRT-PCR analysis of AT2R (B), myogenin (C), and eMyHC (D) in the gastrocnemius muscle after siRNA electroporation on days 0, 3, 5, and 7. Data are mean ± S.E. (n = 5–6). *, p < 0.05; **, p < 0.01.