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Channel properties of OmpF (A) and OmpC (B) mutants. Plasmid-coded porins were expressed in LA51A ΔompF ΔompC, and influx rates of ampicillin and benzylpenicillin were examined under 0 or 300 mm NaCl. Single mutations that give a net positive charge (adding positive charge or removing negative charge) were described in gray type. The others give net negative charge. Co is the substrate concentration used in the assay. Data represent the average of triplicate measurements.
