FIGURE 10.

JH III injection induced BrZ7 expression and phosphorylation and inhibited metamorphosis by suppressing 20E response gene expression. A, phenotypes of DMSO or JH III injection. The 6th-72 h larvae were injected with JH III or 20E (500 ng/larva, 3 × 60 larvae). Scale bar, 1 cm. B, percentage of different phenotypes after JH III injection. C, Western blot showing the BrZ7 expression and phosphorylation by 20E or JH III injection with 7.5% SDS-polyacrylamide gel. Proteins from fat body were obtained at 0, 6, 12, and 24 h after each hormone was injected and analyzed using anti-BrZ7 antibody. D and E, the mRNA levels of various genes were analyzed by qRT-PCR. The RNA of the fat body of 6th-96 h was isolated from 20E- or JH III-injected larvae, respectively. β-actin was used as an internal control sample. Asterisks indicate significant differences (Student's t test; *, p < 0.05). Error bars, S.E.