FIGURE 12.

A summary of the mechanism by which BrZ7 participates in 20E and JH pathways. 20E induces the interaction of EcRB1 with phosphorylated-USP1, regulates BrZ7 high expression, and maintains BrZ7 non-phosphorylation to modulate 20E response gene expression for metamorphosis. JH induces BrZ7 phosphorylation via a GPCR-, PLC-, and PKC-signaling pathway. The phosphorylated BrZ7 directs Cal transcription, and the non-phosphorylated Cal interacts with non-phosphorylated USP1 for larval growth.