FIGURE 7.

qRT-PCR analysis of the function of BrZ7 in JH pathway in HaEpi cells. A, knockdown of BrZ7. Cells were transfected with dsBrZ7 and dsGFP. After 24 h, cells were subjected to JH III for 6 h. RNA was isolated for qRT-PCR. B, overexpression of BrZ7. Cells were transfected with different plasmids, including BrZ7 and its mutants, for 48 h and induced with 1 μm JH III for 6 h. RNA was isolated for qRT-PCR. Results were based on the 2^−ΔΔCT method in the presence of the normalized β-actin gene. Asterisks indicate significant differences (Student's t test; *, p < 0.05). Error bars, S.E.