FIGURE 2.

Catalysis of double-stranded RNA cleavage by human and bovine ribonucleases. A, values k_cat/K_m for the cleavage of poly(A:U). Values (mean ± S.E.) are from at least three independent experiments. **, p < 0.01. B, native polyacrylamide gel showing cleavage of a DNA hairpin containing a single RNA residue (red) and labeled on the 5′ end with FAM (green). SYBR Gold enables imaging of all nucleic acids. C, extent of FAM-labeled product formation for the data in panel B. Values (mean ± S.E.) are from four native gels.