TABLE 1.
Biochemical properties of human and bovine ribonucleases
| Ribonuclease | Molecular mass | Za | Identity to RNase 1 | Similarity to RNase 1b | Tmc | Kdd |
|---|---|---|---|---|---|---|
| kDa | % | °C | fm | |||
| RNase 1 (Homo sapiens) | 14.7 | +6 | 100 | 100 | 55.5 ± 0.5 | 0.12 ± 0.1 |
| BRB (B. taurus) | 15.8 | +11 | 61 | 72 | 52.2 ± 0.4 | 0.35 ± 0.21 |
| mBSR (B. taurus) | 13.7 | +9 | 70 | 80 | 60.1 ± 0.5 | 1.94 ± 0.72 |
| dBSR (B. taurus) | 27.5 | +18 | 70 | 80 | 62e | >2 × 109f |
| RNase A (B. taurus) | 13.7 | +4 | 68 | 82 | 63.9 ± 0.4 | 0.16 ± 0.12 |
a Value is for the net molecular charge: Arg + Lys − Asp − Glu.
b For % similarity calculations: K = R; D = E; C = G = H = N = Q = S = T = Y; A = F = I = L = M = P = V = W.
c Value is the temperature at the midpoint of thermal denaturation, determined by incorporation of a hydrophobic dye and quantitation by differential scanning fluorimetry (33).
d Value is for the complex with bovine ribonuclease inhibitor, determined as described (31, 35, 36).
e Value was determined with circular dichroism spectroscopy (75).
f Value is for the complex with human ribonuclease inhibitor (46).