TABLE 2.
Primers used for SFR2 cloning
F/R means forward/reverse; term is terminal.
| No. | Name | F/R | Sequence (5′ to 3′) |
|---|---|---|---|
| 1 | SFR2 full | F | CACCATGGAATTATTCGCATTGTTAA |
| 2 | SFR2-HIS full | R | AGTCAATGATGATGATGATGATGGCCGTCAAAGGGTGAGGCTAAAG |
| 3 | 27N-term SFR2 | F | TCATGTCTCGTTTCCGTCGCCAGAATCTC |
| 4 | 550C-term His | R | AGTCAATGATGATGATGATGATGTGCGTACATAAGATTATGATTATCAACG |
| 5 | 581C-term His | R | AGTCAATGATGATGATGATGATGACTCAACGGGTCTTGAAGACCATCC |
| 6 | E267A | F | GACTCTTGGGTAACATTTAATGCACCCCATATCTTCACCATG |
| 7 | E267A | R | CATGGTGAAGATATGGGGTGCATTAAATGTTACCCAAGAGTC |
| 8 | E429A | F | GTTCCTTTTATCGTCACAGCAAATGGCGTGTCTGATGAA |
| 9 | E429A | R | TTCATCAGACACGCCATTTGCTGTGACGATAAAAGGAAC |
| 10 | Y377A | F | CATCAACTACGCTGGACAGGAAGCAGTGTG |
| 11 | Y377A | R | CCTATGAAATCCAACTTCTC |
| 12 | I270A | F | GTAACATTTAATGAACCCCATGCTTTCACCATGCTTACCTACATG |
| 13 | I270A | R | CATGTAGGTAAGCATGGTGAAAGCATGGGGTTCATTAAATGTTAC |
| 14 | M273A | F | GAACCCCATATCTTCACCGCTCTTACCTACATGTGTGG |
| 15 | M273A | R | CCACACATGTAGGTAAGAGCGGTGAAGATATGGGGTTC |
| 16 | L274A | F | CCCATATCTTCACCATGGCTACCTACATGTGTGGATC |
| 17 | L274A | R | GATCCACACATGTAGGTAGCCATGGTGAAGATATGGG |
| 18 | I270M273L274A | F | TGCTTTCACCGCAGCCACCTACATGTGTG |
| 19 | I270M273L274A | R | TGGGGTTCATTAAATGTTAC |
| 20 | SFR2N | F | GGAATATTAAGCTCGCCATGGAATTATTCGCATTG |
| 21 | SFR2N | R | GCCTGACTAGCTAACCCAAAGAAGAATTTTC |
| 22 | 4EAM2 | F | GGTTAGCTAGTCAGGCTGGATTCCAG |
| 23 | 4EAM2 | R | TTCTTTGTCCTTGTAGTTTCCCCAGTATCC |
| 24 | SFR2C | F | AACTACAAGGACAAAGAAGTGAAGCTAGC |
| 25 | SFR2C | R | CCGAGGAGAGGGTTATCAATGATGATGATGATGATG |
| 26 | SFR2N_KQF | F | GGAATATTAAGCTCGCCATGGAATTATTCGCATTGTTAAT |
| 27 | SFR2N_KQF | R | TGTCTAGCGAACTGCTTAGCTAACCCAAAGAAGAATTTTCC |
| 28 | ARH_SFR2C | F | AGCAGTTCGCTAGACATGACAAAGAAGTGAAGCTAGC |
| 29 | ARH_SFR2C | R | CCGAGGAGAGGGTTATCAATGATGATGATGATGATG |