FIGURE 1.

ego− mutant defect is selective for recovery from rapamycin. Cultures of WT, ego1Δ, or gtr2Δ cells in mid-logarithmic phase were diluted to an A_{600 nm} of ∼0.1 and treated or not for 2 h with rapamycin (200 ng/ml) or caffeine (9 mm) or deprived of carbon or nitrogen for 2 h at room temperature. Samples were washed in fresh YPD medium, and 10-fold serial dilutions were spotted (5 μl) onto YPD plates and incubated at 30 °C for 2 days prior to scanning.