FIGURE 5.

Residual growth rate seen in the presence of rapamycin persists after washout of the drug. A, logarithmically growing cultures of WT cells were diluted to an A_{600 nm} of ∼0.1 and treated or not with rapamycin (40 ng/ml) for 2 h at room temperature after which cells were washed in fresh medium and transferred to fresh medium to recover at room temperature. Cell number was monitored by Coulter counting over the course of 12 h. The log₂ of the increase in cell number relative to t = 0 (i.e. time of drug washout) was determined, and trend lines were plotted. Error bars denote S.E. B, logarithmically growing cultures of ego1Δ cells were treated and analyzed as for A.