FIGURE 4.

Appearance and skeletal system of Galnt3 transgenic embryos. A, macroscopic appearance of wild-type (wt) and Galnt3 transgenic (tg) embryos at E18.5. The abdomen in Galnt3 transgenic embryos was protruded because of the small thoracic cage. B, macroscopic views of the palates of wild-type (left) and Galnt3 transgenic (right) embryos at E18.5. A Galnt3 transgenic embryo presents cleft palate (arrow). C, skeletons of wild-type (left) and Galnt3 transgenic (right) embryos at E18.5 stained with alizarin red and Alcian blue. The arrows indicate the reduced Alcian blue staining and the lack of alizarin red staining in the bones of the hand and foot. Scale bars, 1 mm.