FIGURE 6.

In situ hybridization and real-time RT-PCR analysis. Shown is in situ hybridization of sections of tarsal and metatarsal bones from wild-type (A, C, E, and G) and Galnt3 transgenic (B, D, F, and H) embryos at E18.5 using Col2a1 (A and B), Acan (C and D), Col10a1 (E and F), and Spp1 (G and H) antisense probes. We detected no signal using sense probes (data not shown). Scale bar, 100 μm. I, real-time RT-PCR analysis of Galnt3, Ihh, Pthlh, Pth1r, and Bmp2. RNA was extracted from the cartilaginous tissues of four wild-type and four F₀ Galnt3 transgenic embryos at E15.5.