FIGURE 10.

Activation of the NF-κB pathway by PARP12. HEK293T cells were transfected with the indicated constructs of PARP12 and assayed for A, luciferase expression when co-transfected with an NFκB-sensitive promoter (pNFκB-Luc, Firefly) and a control plasmid encoding for a Renilla Luciferase (pGL4.74). The data represent the mean ± S.E. of the ratio of Firefly/Renilla activity normalized to the sample not transfected with PARP12 (***: p value < 0.001, Friedman test with Dunn's multiple comparisons) and B, IL-8 secretion upon stimulation with rhTNF (10 ng/ml). The data represent mean ± S.E. of four independent experiments (**: p value < 0.01 - Kruskal-Wallis test with Dunn's multiple comparisons. C, subcellular localization of the different mutants of PARP12 in HeLa cells. The NFκB-activating mutants of PARP12 spontaneously form cytoplasmic puncta.