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. 2014 Jul 10;42(16):e127. doi: 10.1093/nar/gku604

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© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Design of Cre cassettes. (A) n sequence fragments, each flanked by loxP sites (black triangles) in opposing orientations are concatenated to form a cassette. Upon Cre recombination, the cassette is shuffled and pared down until a single fragment, j, remains in either orientation. (B) Several variant mutually incompatible lox sites have been described, each of which has a pair of mutations in the spacer sequence. (C) The diversity attainable by Cre recombination can be increased by concatenating k cassettes, where each cassette utilizes an incompatible lox site. Cre recombination results in the independent shuffling of each of the k cassettes. (D) The diversity as a function of length for cassettes containing n = 100 fragments (100bp each) concatenated together. Lengths >60kb are needed to reach the requisite diversity of 10⁹. (E) The distribution of the number of recombination events needed for each cassette to reach completion.